ERCC6L Antibody

Catalog Number:

36448

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q2NKX8
NCBI Gene ID: 54821
Gene Accssion: BC008808

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to residues near the C terminal of human excision repair cross-complementation group 6-like

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total ERCC6L protein.

Applications:

IHC

Background:

PICH (Plk1-interacting checkpoint helicase), also known as DNA excision repair protein ERCC-6-like (ERCC6L) or tumor antigen BJ-HCC-15, is a 1,250 amino acid protein belonging to the SNF2/RAD54 helicase family. PICH is a DNA helicase and an essential component of the spindle assembly checkpoint. During mitosis, PICH recruits MAD2 to kinetochores and also regulates the tension on centromic chromatin. PICH is concentrated in between the kinetochores in prometophase cells, while in metaphase it localizes to the thin threads composed of catenated centromeric DNA that stretch between sister kinetochores. PICH is phosphorylated by Plk, which prevents PICH from associating with chromosome arms and restricts the localization of PICH to the kinetochore-centromere region. PICH/Plk interaction is also required for correct Plk localization to the kinetochore. PICH contains one helicase ATP-binding domain, two TPR repeats and one helicase C-terminal domain.

References:

appl_detail:

Immunohistochemistry: 1:50-1:200

Datasheet PDF