IDH3B Antibody

Catalog Number:

36157

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O43837
NCBI Gene ID: 3420
Gene Accssion: BC001960

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to a region derived from internal residues of human isocitrate dehydrogenase 3 (NAD+) beta

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total IDH3B protein.

Applications:

WB IHC

Background:

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the beta subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. Three alternatively spliced transcript variants encoding different isoforms have been described for this gene.

References:

appl_detail:

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100

Datasheet PDF