ACAD11 Antibody

Catalog Number:

36016

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q709F0
NCBI Gene ID: 84129
Gene Accssion: BC125204

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to a region derived from internal residues of human Acyl-CoA dehydrogenase family, member 11

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total ACAD11 protein.

Applications:

WB IHC

Background:

The deduced full-length?ACAD11?protein contains an N-terminal aminoglycoside phosphotransferase domain, followed by a mitochondria localization signal and ACAD N-terminal, middle, and C-terminal domains. It also has a glycosylation site and a C-terminal peroxisome-targeting signal. The ACAD domain contains a catalytic aspartate rather than the catalytic glutamate found in most other ACADs. Real-time RT-PCR detected variable?ACAD11?expression in all tissues examined, with highest expression in adult brain, followed by heart, liver, spinal cord, and kidney. Western blot analysis of fractionated human cerebellum and kidney revealed thatACAD11?associated with mitochondrial membranes, but not mitochondrial matrix. Immunohistochemical analysis showed that?ACAD11colocalized with a mitochondrial marker in human neuroblastoma cells.

References:

appl_detail:

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:15-1:50

Datasheet PDF