SPIN2B Antibody

Catalog Number:

35931

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: Q9BPZ2
NCBI Gene ID: 474343
Gene Accssion: BC000044

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Full length fusion protein

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total SPIN2B protein.

Applications:

WB IHC

Background:

Human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal, but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin. On the contrary, SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death. Also, SPIN2 did not combat the proapoptotic effects of Fas ligand stimulation in the Jurkat human T-cell line. Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity. In the murine myeloid cells, SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth. There was also an increased percentage of cells in G2/M, which was more pronounced following IL3 withdrawal.

References:

appl_detail:

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100

Datasheet PDF