DFFA Antibody

Catalog Number:

35774

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O00273
NCBI Gene ID: 1676
Gene Accssion: BC007721

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to residues near the N terminal of human DNA fragmentation factor, 45kDa, alpha polypeptide

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total DFFA protein.

Applications:

IHC

Background:

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.?

References:

appl_detail:

Immunohistochemistry: 1:10-1:100

Datasheet PDF