MGEA5 Antibody

Catalog Number:

35763

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot#: O60502
NCBI Gene ID: 10724
Gene Accssion: BC039583

Form of Antibody:

Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage/Stability:

Immunogen:

Fusion protein corresponding to a region derived from internal residues of human Meningioma expressed antigen 5 (hyaluronidase)

Purification:

Antigen affinity purification.

Specificity/Sensitivity:

The antibody detects endogenous levels of total MGEA5 protein.

Applications:

WB IHC

Background:

The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT, which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications. Cleaves GlcNAc but not GalNAc from glycopeptides. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc. Possesses hyaluronidase activity. Acetylates 'Lys-8' of histone H4 and 'Lys-14' of histone H3. Shows highest expression in the brain, placenta and pancreas.

References:

appl_detail:

Western blotting: 1:200-1:1000
Immunohistochemistry: 1:25-1:100

Datasheet PDF