SLC27A5 Antibody

Catalog Number:

34498

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q9Y2P5
NCBI Gene ID: 10998

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human SLC27A5.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total SLC27A5 protein.

Applications:

WB IHC IF

Background:

Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation. In vitro, also activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol.

Steinberg S.J., Mol. Genet. Metab. 68:32-42(1999).
Steinberg S.J., J. Biol. Chem. 275:15605-15608(2000).
Mihalik S.J., J. Biol. Chem. 277:24771-24779(2002).

References:

appl_detail:

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500

Datasheet PDF