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ATP5I Antibody

Catalog Number:

34448
other_names: ATP synthase e chain; mitochondrial; ATP5K; EC 3.6.3.14; F1F0-ATP synthase

Amount:

100μg
calculated_mw:
host_species: Rabbit

Price:

$319

Swiss-Prot No:

Swiss-Prot: P56385
NCBI Gene ID: 521

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from C-terminal of human ATP5I.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total ATP5I protein

Applications:

WB

Background:

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F0 domain. Minor subunit located with subunit a in the membrane.

Fujiwara T., Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
Kalnine N., Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
The MGC Project Team, Genome Res. 14:2121-2127(2004).

References:

appl_detail:

Western blotting: 1:500~1:3000

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