ASAH3L Antibody

Catalog Number:

34404

Amount:

100μg

Price:

$319

Swiss-Prot No:

Swiss-Prot: Q5QJU3
NCBI Gene ID: 340485

Form of Antibody:

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage/Stability:

Immunogen:

Synthesized peptide derived from internal of human ASAH3L.

Purification:

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Specificity/Sensitivity:

The antibody detects endogenous levels of total ASAH3L protein.

Applications:

WB

Background:

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. The substrate preference is D-erythro-C(18:1)-, C(20:1)-, C(20:4)-ceramide > D-erythro-C(16:0)-, C(18:0), C(20:0)-ceramide > D-erythro-C(24:1)-ceramide > D-erythro-C(12:0)-ceramide, D-erythro-C(14:0)-ceramides > D-erythro-C(24:0)-ceramide > D-erythro-C(6:0)-ceramide. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate.

Xu R., FASEB J. 20:1813-1825(2006) [PubMed: 16940153].
Wan D.,Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004) [PubMed: 15498874].
Humphray S.J., Nature 429:369-374(2004) [PubMed: 15164053].

References:

appl_detail:

Western blotting: 1:500~1:3000

Datasheet PDF