Product Datasheet  
NCK1 Antibody  
Catalog Number: 49743  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#:P16333
  • Form of Antibody：  
  • 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • Recombinant protein
  • reactivity：  
  • Hu, Ms, Rt
  • appl_detail：  
  • WB: 1:500-1:2,000
    IHC: 1:50-1:200
    ICC: 1:50-1:200
    IP: 1:10-1:50
    FC: 1:50-1:100
  • other_names：  
  • Cytoplasmic protein NCK1 antibody
    Melanoma Nck protein antibody
    MGC12668 antibody
    NCK 1 antibody
    NCK adaptor protein 1 antibody
    NCK alpha antibody
    NCK antibody
    NCK tyrosine kinase antibody
    Nck-1
  • Purification：  
  • ProA affinity purified
  • Specificity：  
  • Applications：  
  • WB,ICC,IF,IHC,IP,FC
  • Background：  
  • The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr 1009); overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q22.3 and 2q12.2, respectively.



 
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