Product Datasheet  
Erk1 / Erk2 (Phospho-Thr 202) Antibody  
Catalog Number: 13326  
Technical:tech@swbio.com  
Information:info@swbio.com  
Description  
  • host_species：  
  • Rabbit
  • Amount：  
  • 100μgμg
  • Swiss-Prot No.：  
  • Swiss-Prot#:P27361
  • Form of Antibody：  
  • 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage：  
  • Store at -20˚C
  • Immunogen：  
  • A short amino acid sequence containing phosphorylated Thr 202 of ERK 1/2 of human origin
  • reactivity：  
  • Hu, Ms, Rt
  • appl_detail：  
  • WB: 1:100-1:1,000
    IHC: 1:50-1:500

    IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
  • other_names：  
  • ERK 1 antibody
    ERK 2 antibody
    ERK-2 antibody
    ERK1 antibody
    erk1/2 antibody
    ERK2 antibody
    ERT1 antibody
    ERT2 antibody
    Extracellular signal regulated kinase 1 antibody
    Extracellular signal-regulated kinase 2 anti
  • Purification：  
  • Immunogen affinity purified
  • Specificity：  
  • Applications：  
  • WB, IP, IF, IHC(P)
  • Background：  
  • The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively (1C3). ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif (4,5). Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK1 and Threonine 185 and Tyrosine 187 residues of ERK2 is required for full enzymatic activation (6). The structural consequences of dual-phosphorylation in the ERK2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop (6). In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (4,5). Upstream MAP kinase regulators include MAP kinase kinase (MEK), MEK kinase and Raf-1 (5). The ERK family has three additional members: ERK 3, ERK 5 and ERK 6 (7,8).



 
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